koustav_sarkar.jpg

Dr. Koustav Sarkar

Ph.D

Research Assistant Professor


Dr. Koustav Sarkar is working as a Research Assistant Professor in SRM Research Institute and associated with the Department of Biotechnology at SRM Institute of Science and Technology (formerly known as SRM University), since April 2016. His research focuses on explicating the molecular underpinnings of immune dysregulation and cancer immunology immunotherapy.

Research Contribution

Dr. Sarkar has been involved in research over the last fourteen years (including a Ph.D. and three Post-Docs) and made several important contributions to the development of advanced science and technology. He was involved in understanding the molecular mechanisms of the development of human immune responses in health & disease. He has already published 25 high-impact scientific publications in internationally reputed journals with citation indices 437 and h-index 13. He is also co-author of four book chapters and fourteen conference proceedings. During Ph.D., Dr. Sarkar has developed a process for isolating glycoprotein(s) from neem leaf, which has immunomodulatory and cancer preventive functions. One patent (Patent Number: 259434; Grant Date: 12-Mar-2014) has been granted for this invention. He found out that the neem leaf glycoprotein helped to generate carcinoembryonic antigen specific anti-tumor immune responses utilizing macrophage & dendritic cell mediated antigen presentation to T and B cells and the induction of type 1 protective immunity. To study the intricate molecular mechanisms involved in the type 1 protective immunity, Dr. Sarkar moved to USA. Research from his US laboratory was essential in revealing for the first time a novel nuclear function for a well known cytoskeleton structure associated protein, Wiskott Aldrich Syndrome Protein (WASp) in the transcriptional regulation of T helper cell 1 (Th1)-differentiation through its effect on epigenetic modifications at the T-BET gene-promoter locus. Since that time, Dr. Sarkar has been actively involved in further understanding how WASp associates with and regulates protein pathways and gene networks that control development of protective type-1 immunity.

Academic Profile

Education:


Institution and Location

Degree

   Year

Banaras Hindu University, Varanasi, India

MSc

2002

Jadavpur University, Kolkata, India

PhD

2009

Area of Specialization

  • Immunology, Cancer Biology, Epigenetics

Professional Career

August 14, 2002 – Present

  • Research Assistant Professor (SRM Institute of Science and Technology (formerly known as SRM University), Kattankulathur, Tamil Nadu – 603203, India) [April 01, 2016 – Present]  
  • Post-Doctoral Research Scholar (University of Iowa Children’s Hospital, Iowa City, USA) [June 30, 2013 – March 25, 2016]
  • Post-Doctoral Associate (Children’s Hospital of Pittsburgh, Pittsburgh, USA) [Sept 15, 2009 – June29, 2013] 
  • Post-Doctoral Research Fellow (Massachusetts General Hospital, Harvard University, Boston, USA) [Feb 02, 2009 - Sept 14, 2009] 
  • Senior Research Fellow (Indian Council of Medical Research) [Dec 1, 2005 - Jan 30, 2009]
  • Junior Research Fellow (Department of Science and Technology, Govt. of India) [Mar 01, 2003 - Jul 24, 2005] 
  • Project Fellow (University Grants Commission, India) [Aug 14, 2002 - Feb 28, 2003]

Awards

  1. DST-SERB Early Career Research Award – A grant of ~Rs. 45 lakhs
  2. American Association of Immunologists (AAI) 2015 Trainee Abstract Award

Achievements

  • One of the works was chosen for perspective of Science Translational Medicine 2, 37ps31, 2010: “Alternative control: Whatʼs WASp doing in the nucleus?” by Teitell, M. A.
  • One of our experimental figures was chosen for the ONLINE COVER of Science Translational Medicine 23 June 2010 as “A Closer Look at Wiskott-Aldrich syndrome” 
  • The research work was also cited in The Money Times as “Study identifies disease protein” [http://www.themoneytimes.com/20100623/study-identifies-disease-protein-id- 10118440.html]. Eureka Alert described our findings as “The Sting of WASp in Immune Disorders”[http://www.eurekalert.org/multimedia/pub/23428.php].
  • The work was also narrated in Ecoworld as “Study identifies disease protein” [http://www.ecoworld.com/?s=Study+identifies+disease+protein&x=0&y=0&=Go]
  • A work was cited as “Non-toxic Cancer Treatment” in the website of Technology Innovation Management & Entrepreneurship Information Service (TIMEIS) of DST, Govt. of India 
  • Another work was discussed in the home page of Food and Agriculture Organization of the United Nations under the sub-heading “Other publications of interest”

Fellowships

  • Post-Doctoral Research Scholarship from National Institutes of Health National Institute of Allergy and Infectious Diseases, USA funded two R01 grants (#AI07356 and #AI084957) (September 15, 2009 – March 25, 2016)
  • Post-Doctoral Research Fellowship from MGPO, USA funded grant (February 2, 2009 – September 14, 2009)
  • Senior Research Fellowship from ICMR, India sponsored project (December 1, 2005 – January 30, 2009)
  • Junior Research Fellowship from DST, India sponsored project (March 1, 2003 – July 27, 2005)
  • Project Fellowship from UGC, India sponsored project (August 14, 2002 – February 28, 2003)

Membership of professional bodies

  • Member of The American Association of Immunologists
  • Life Member of Indian Science Congress
  • Life Member of Indian Association for Cancer Research
  • Life Member of Indian Immunology Society

Invited Talks:

  • Sarkar K. Wiskott Aldrich Syndrome protein (WASp) mediated epigenetic regulation of T helper (Th) cell differentiation linked to malignancy through R-loop modulation via magnesium dependent calorie restriction. Invited talk at CSIR-Central Food Technological Research Institute (CFTRI), Mysore, Karnataka, India on January 18, 2019.
  • Sarkar K. Wiskott Aldrich Syndrome protein (WASp) mediated epigenetic regulation of T helper (Th) cell differentiation linked to malignancy through R-loop modulation using CRISPR/Cas9 gene editing technology. Invited talk in a seminar organized by Special Interest Group (SIG) on Cancer Biology and Cancer Stem Cells (CBCSC) at JSS Medical College, JSS Academy of Higher Education and Research, Bannimantapa, Sri Shivarathreeshwara Nagar, Mysore, Karnataka, India on January 17, 2018.
  • Sarkar K. CRISPR/Cas9: Mechanism to applications in genome editing. Delivered a talk as an invited expert speaker for a two day workshop on “Gene editing using “CRISPR-CAS”: Hands on training on its basics and applications in drug discovery” Organised by TIFAC CORE in HD Department of Pharmacognosy & Phytopharmacy JSS College of Pharmacy, Ooty in association with CEMR Laboratory JSS Medical College, Mysuru, Karnataka & Department of Pharmaceutical Bio-Technology, JSS College of Pharmacy, Ooty during December 13-14, 2018.
  • Sarkar K. CRISPR/Cas9 based epigenome editing. Delivered a talk as an invited expert speaker for a two day workshop on “Gene editing using “CRISPR-CAS”: Hands on training on its basics and applications in drug discovery” Organised by TIFAC CORE in HD Department of Pharmacognosy & Phytopharmacy JSS College of Pharmacy, Ooty in association with CEMR Laboratory JSS Medical College, Mysuru, Karnataka & Department of Pharmaceutical Bio-Technology, JSS College of Pharmacy, Ooty during December 13-14, 2018.
    Sarkar K. Wiskott Aldrich Syndrome protein (WASp) mediated epigenetic regulation of T helper (Th) cell differentiation linked to malignancy through R-loop modulation. Delivered a talk as an invited speaker in 7th International Conference on Translational Cancer Research organized by IIT Madras and sponsored by The University of Texas MD Anderson Cancer Center during February 8 -11, 2018, at Chennai, India.
  • Sarkar K. Sumoylation switches Wiskott Aldrich Syndrome Protein (WASp) from a transcriptional activator to a repressor. Invited talk at CSIR-Central Drug Research Institute, Lucknow, UP, India on November 27, 2015.
  • Sarkar K, Sadhukhan S, Han SS, Vyas YM. Disruption of hSWI/SNF-Complexes in T cells by WAS Mutations Distinguishes X-linked Thrombocytopenia from Wiskott-Aldrich Syndrome. Invited for oral presentation at 4th Annual Retreat of Center for Immunology and Immune-based Diseases, Iowa City, IA, USA on August 20, 2015.
  • Sarkar K, Sadhukhan S, Han SS, Vyas YM. Nucleosomal Remodeling Defects at Gene Promoters Underlie Cross-Phenotype Effects in X-linked Thrombocytopenia/Wiskott-Aldrich Syndrome. Invited for oral presentation at Block symposium of American Association of Immunologists (AAI) Annual Meeting 2015, New Orleans, LA, USA, May 8-12, 2015.
  • Sarkar K, Sadhukhan S, Han SS, Vyas YM. Disruption of hSWI/SNF-Complexes in T cells by WAS Mutations Distinguishes X-linked Thrombocytopenia from Wiskott-Aldrich Syndrome. Invited for oral presentation on Pediatric Research Day symposium 2015, Iowa City, IA, USA.

Journal Reviewer

  • “Journal of Oncology” by Hindwai Publishing Corporation
  • “Applied Surface Science” by Elsevier
  • “BMC Immunology” by BioMed Central
  • “Journal of Mines, Metals and Fuels” by Books & Journals Private Ltd.

Research Interest

  • Cancer Immunology & Immunotherapy
  • Epigenetic regulation

 

Leader

  • Dr. Koustav Sarkar

Postdoctoral Fellows

  • To be updated

Research Fellows

  • Mr. Rajib Dhar [SRM Institute of Science and Technology sponsored full time Research Scholar] (PhD. Student)
  • Ms. Sukanya Mani [Junior Research Fellow in SERB, DST sponsored project]
  • Ms. Sudeshna Rakshit [SRM Institute of Science and Technology sponsored full time Research Scholar]

Project Assistants

  • To be updated

Selected publications

  • Sarkar K, Han SS, Wen KK, Ochs HD, Dupre L, Seidman MM, Vyas YM. R-loops cause genomic instability in T helper lymphocytes from patients with Wiskott-Aldrich syndrome. J Allergy Clin Immunol, 142, 219-234, 2018. Impact factor: 13.258.Cited by related articles: 2.
  • Ghosh T, Barik S, Bhuniya A, Dhar J, Ghosh S, Sarkar M, Guha I, Sarkar K, Chakrabarti P, Saha B, Storkus WJ, Baral R, Bose A. Tumor-associated mesenchymal stem cells inhibit naïve T cell expansion by blocking cysteine export from dendritic cells. Int J Cancer, 139, 2068-2081, 2016. Impact factor: 7.36. ISSN: 1097-0215. Cited by related articles: 2.
  • Sarkar K, Sadhukhan S, Han SS, Vyas YM. SUMOylation-disrupting WAS Mutation Converts WASp from a Transcriptional Activator to a Repressor of NF-κB Response Genes in T cells. Blood, 126, 1670-1682, 2015. Impact factor: 15.132. ISSN: 0006-4971. Cited by related articles: 13.
  • Sarkar K, Sadhukhan S, Han SS, Vyas YM. Disruption of hSWI/SNF-Complexes in T cells by WAS Mutations Distinguishes X-linked Thrombocytopenia from Wiskott-Aldrich Syndrome. Blood, 124, 3409-3419, 2014. Impact factor: 15.132. ISSN: 0006-4971. Cited by related articles: 13.
  • Sadhukhan S*, Sarkar K*, Taylor M, Candotti F, Vyas YM. Nuclear Role of WASp in Gene Transcription is Uncoupled from its ARP2/3-Dependent Cytoplasmic Role in Actin Polymerization. *Both authors contributed equally to this paper. J Immunol, 193, 150-160, 2014. Impact factor: 5.36. ISSN: 0022-1767. Cited by related articles: 26.
  • Taylor MD, Sadhukhan S, Kottangada P, Ramgopal A, Sarkar K, DʼSilva S, Selvakumar A, Candotti F, Vyas YM. Nuclear Role of WASp in the Pathogenesis of Dysregulated TH1-Immunity in Human Wiskott-AldrichSyndrome. SciTransl Med (Science AAAS), 2, 37ra44, 2010. Impact Factor: 16.796. ISSN: 1946-6242. Cited by related articles: 86.
  • Sarkar K, Goswami S, Roy S, Mallick A, Chakraborty K, Bose A, Baral R. Neem leaf glycoprotein enhances carcinoembryonic antigen presentation of dendritic cells to T and B cells for induction of antitumor immunity by allowing generation of immune effector/memory response. Int Immunopharmacol, 10, 865-874, 2010. Impact Factor: 2.659. ISSN: 1567-5769. Cited by related articles: 27.
  • Sarkar K, Bose A, Haque E, Chakraborty K, Chakraborty T, Goswami S, Ghosh D, Baral R. Induction of type 1 cytokines during neem leaf glycoprotein assisted carcinoembryonic antigen vaccination is associated with nitric oxide production. Int Immunopharmacol, 9, 753-760, 2009. Impact Factor: 2.659. ISSN: 1567-5769. Cited by related articles: 14.
  • Sarkar K, Bose A, Chakraborty K, Haque E, Ghosh D, Goswami S, Chakraborty T, Laskar S, Baral R. Neem leaf glycoprotein helps to generate carcinoembryonic antigen specific antitumor immune responses utilizing macrophage mediated antigen presentation. Vaccine, 26, 4352-4362, 2008. Impact Factor: 3.492. ISSN: 0264-410X. Cited by related articles: 34.
  • Sarkar K, Bose A, Laskar S, Chaudhuri SK, Dey S, Roychoudhuri PK, Baral R. Antibody response against neem leaf preparation recognizes carcinoembryonic antigen. Int Immunopharmacol, 7, 306-312, 2007. Impact Factor: 2.659. ISSN: 1096- 9861. Cited by related articles: 28.

Book Chapter:

  • Bose A, Goswami S, Roy S, Sarkar K, Chakraborty K, Chakraborty T, Haque E, Laskar S, Baral R. Immunotherapeutic Targeting of Multiple Dysregulated Immune Functions in Cancer by Neem Leaf Glycoprotein. In: Natural Products: Research Reviews (Ed. V. K. Gupta) Vol 1, Chap 2, Daya Publishing House, 2012. ISBN 13 9788170357759; ISBN 10 8170357756.
  • Baral RN, Sarkar K, Mandal-Ghosh I, Bose A. Neem leaf glycoprotein as a new vaccine adjuvant for cancer immunotherapy. In: “Comprehensive Bioactive Natural Products; Immune-modulation & Vaccine Adjuvants” (Ed. V. K. Gupta), Vol 5, Chap 2, Studium Press LLC, USA, 2010, pp. 21-45. ISBN-10 1933699558.
  • Bose A, Chakraborty K, Sarkar K, Goswami S, Chakraborty T, Haque E, Ghosh D, Mandal- Ghosh I, Pal S, Laskar S, Chattopadhyay U and Baral RN. Cancer Immunoediting: Prefessional Editorship by Neem leaf glycoprotein. In: “Treatments of Advanced Stage Cancers: current status and emerging frontiers” (Ed. G. P. Talwar & O. P. Sood) by Narosa Publishing House Pvt. Ltd, India, 2009, pp 148-152. ISBN 10: 8173199329; ISBN 13: 9788173199325.
  • Chakraborty T, Bose A, Chakraborty K, Sarkar K, Goswami S, Haque E, Ghosh D, Mandal- Ghosh I, Pal S, Laskar S and Baral RN. Suppression of Suppressors: Neem leaf glycoprotein Guided Crosstalk Between Regulatory T cells and T cells/NK Cells/ Macrophages in Cancer. In: “Treatments of Advanced Stage Cancers: current status and emerging frontiers” (Ed. G.P. Talwar & O. P. Sood) by Narosa Publishing House Pvt. Ltd, India, 2009, pp 153-157. ISBN 10: 8173199329; ISBN 13: 9788173199325.

Patents

  • Baral RN, Laskar S, Bose A, Sarkar K, Process for isolating glycoprotein from neem leaf and its characterization to define the immunomodulatory and cancer preventive functions of this glycoprotein. Patent Number: 259434; Indian Patent Application Number: 429/KOL/2007; Publication Date: 14-Mar-2014; Grant Date: 12-Mar-2014.

Academic Experience

  • Teaching Responsibilities: I am involved in teaching of B.Tech. and M.Tech. students (April 01, 2016 - Present)

Administrative Responsibilities

  • Curriculum and Syllabi: I am involved in making Curriculum and Syllabi
  • Awareness and Coaching of students for abroad and local scholarship programs such as MITACS and GATE: I guide and recommend the students for such programs
  • As counselor: I advice and guide the students for placement and competitive exams

Research Experience

  • August 14, 2002 – Present

Research Assistant Professor

  •  (SRM Research Institute, SRM University, Kattankulathur, Tamil Nadu – 603203, India) [April 01, 2016 – Present]

Post-Doctoral Research Scholar

  •  (University of Iowa Children’s Hospital, Iowa City, IA 52246, USA) [June 30, 2013 – March 25, 2016]

Post-Doctoral Associate

  •  (Children’s Hospital of Pittsburgh, Pittsburgh, PA 15213, USA) [Sept 15, 2009 – June29, 2013]

Post-Doctoral Research Fellow

  • (Massachusetts General Hospital, Harvard University, Boston, MA 02114, USA) [Feb 02, 2009 - Sept 14, 2009]

Senior Research Fellow

  •  (Indian Council of Medical Research) [August 01, 2005 - Jan 30, 2009]

Junior Research Fellow

  •  (Department of Science and Technology, Govt. of India) [Mar 01, 2003 - Jul 27, 2005]

Project Fellow

  • (University Grants Commission, India) [August 14, 2002 - February 28, 2003]

Research Guidance

  • PhD Students: 2 (pursuing)
  • M.Tech. Students: 2 (completed); 1 (pursuing)
  • B.Tech. Students: 8 (completed major projects); 15 (completed minor projects and Industrial Training; 3 (pursuing major projects)

Achievements and awards

 Grant

  • Recipient of SERB sponsored Early Career Research Award (ECRA), 2016

Fellowship(s)/Scholarship(s)

  • Post-Doctoral Research Scholarship from National Institutes of Health National Institute of Allergy and Infectious Diseases, USA funded two R01 grants (#AI07356 and #AI084957) (September 15, 2009 – March 25, 2016)
  • Post-Doctoral Research Fellowship from MGPO, USA funded grant (February 2, 2009 – September 14, 2009)
  • Senior Research Fellowship from ICMR, India sponsored project (December 1, 2005 – January 30, 2009)
  • Junior Research Fellowship from DST, India sponsored project (March 1, 2003 – July 27, 2005)
  • Project Fellowship from UGC, India sponsored project (August 14, 2002 – February 28, 2003)

Conference

  • American Association of Immunologists (AAI) 2015 Trainee Abstract Award at AAI Annual Meeting – IMMUNOLOGY 2015 – held May 8-12, in New Orleans, LA, USA.

Funded Projects

As Principal Investigator (PI):

  • Title of the project: “Wiskott Aldrich Syndrome protein (WASp) mediated epigenetic regulation of T helper (Th) cell differentiation linked to malignancies through R-loop modulation”.Funding Agency: SERB, DST under “Early Career Research (ECR) Award” Date of Start: March 03, 2017; Duration: 36 months; Sanctioned amount: Rs. 44,45,210/-
  • As Co-PI:Title of the project: “Therapeutic potential of Nrf2 activators on endoplasmic reticulum stress-induced endothelial dysfunction in diabetes”.Funding Agency: SERB, DST under “Extra Mural Research Funding” Date of Start: October 09, 2017, Duration: 36 months; Sanctioned amount: Rs. 50,54,200/-

Other professional activities

Reviewer

  • “Applied Biochemistry and Biotechnology” by Springer
  • “Applied Surface Science” by Elsevier
  • “BMC Immunology” by BioMed Central
  • “Journal of Oncology” by Hindwai Publishing Corporation
  • “Journal of Mines, Metals and Fuels” by Books & Journals Private Ltd.

Convener for Seminars/Workshops

  • Convener of seminar series in the Department of Biotechnology at SRM Institute of Science and Technology during 2017-2018.
  • Convener of seminar on “Understanding viral oncogenesis using HTLV as a model” to celebrate World Day of Immunology in association with Indian Immunology Society on April 29, 2017.
  • Convener of “Workshop on Flowcytometry” to celebrate World Day of Immunology in association with Indian Immunology Society on May 02, 2018.

 Organizing committee member for International/National conferences

  • Asis-Oceania Sonochemical Society (AOSS-3) International Conference at SRM Research Institute, SRM University, Chennai, India, September 14-16, 2017.
  • 104th Indian Science Congress at Sri Venkateswara University, Tirupati, india, January 3-7, 2017.

Member of professional bodies, societies

  • Member of the American Association of Immunologists (AAI)
  • Life Member of Indian Science Congress Association (ISCA)
  • Life Member of Indian Association for Cancer Research (IACR)
  • Life Member of Indian Immunology Society (IIS)

Completion of online courses

  • Successfully qualified the National Programme on Technology Enhanced Learning (NPTEL) conducted online course: Introduction to Proteomics in Elite category in November, 2016.

 

 

2016 Odd Semester [July – November]

  • M. Tech. Biotechnology: Semester III; Course Name: Stem Cell Technology

2017 Even Semester [January – May]

  • B. Tech. Biotechnology: Semester VI; Course Name: Stem Cell Biology
  • B. Tech. Biotechnology: Semester IV; Course Name: Immunology

2017 Odd Semester [July – November]

  • B. Tech. Biotechnology: Semester V; Course Name: Ethical Issues, Research Methodology and Intellectual Property Rights
  • B. Tech. Biotechnology: Semester III; Course Name: Cell Biology Laboratory
  • B. Tech. Biotechnology: Semester I; Course Name: Biology for Engineers

2018 Even Semester [January – May]

  • B. Tech. Biotechnology: Semester VI; Course Name: Stem Cell Technology
  • B. Tech. Biotechnology: Semester IV; Course Name: Vaccine Biotechnology
  • B. Tech. Biotechnology: Semester IV; Course Name: Molecular Biology Laboratory

2018 Even Semester [July – November]

  • B. Tech. Biotechnology: Semester VII; Course Name: Cancer Biology
  • B. Tech. Biotechnology: Semester III; Course Name: Cell Biology Laboratory

 

Postdoctoral Positions

  • Will be updated soon

Project Assistants

  • Will be updated soon

Contact

Dr. Koustav Sarkar
DST-SERB Early Career Researcher
Research Assistant Professor
Research Institute and Department of Biotechnology
SRM Institute of Science and Technology (formerly known as SRM University), Kattankulathur-603203, Tamilnadu, India
Email: koustavm@srmist.edu.in
Phone: 044-2471 7908